Cellular and systemic effects of pulsed Nd:YAG laser - Hilterapia

(I-Newswire) March 15, 2010 - Notwithstanding the wide diffusion of lasertherapy in clinics and numerous studies reported in literature, molecular mechanisms of interaction between laser and tissues are not well understood. The analysis of biological effects induced by laser radiation is rather complicated due to the wide possibility of setting instruments, the variability of applied protocols and the differences in treated tissues.

Starting from studies on photothermal effects, the hypothesis is that this type of laser cause an indirect photomechanical effect. The heat produced by transfer of radiation energy to the irradiated volume, diffuse into surrounding tissues, inducing temperature gradients which result in transitory modifications of mechanicalelastic properties of the extracellular microenvironment, thus changing mechanical forces acting on cells. Considering these studies and knowing the key role of the extracellular matrix, not only as a structural support but also in maintaining tissue homeostasis, our experiments focused on the analysis of extracellular matrix molecules and cytoskeleton behavior, responsible of contact between cell and matrix and considered the best candidate to act as a mechanotrasducer.

The data obtained have shown, in laser-treated cells, an increase in production of EC M molecules, such as aggrecan, collagen I and II, and a reorganization of microtubules and actin microfilaments network. It is well know that similar effects are obtained when cells are subjected to mechanical stress. Our data on absorption of Nd:YAG pulses by matrix components (proteins and polysaccharides) suggest that Nd:YAG pulses principally interact with the extracellular matrix, whose transitory deformation applies a mechanical stress to the cells. We then focused on the effect of pulsed Nd:YAG on endothelial function and tissue repair processes. In treated endothelial cells and fibroblasts, key elements of angiogenesis and tissue repair, we found overexpression of genes involved in the chemokine mediated inflammatory pathways. Moreover, the treatment promoted the formation of ordered endothelial monolayers as well as ordered fibronectin fibril assembling. The findings indicate that treatment with Nd:YAG pulses has a stimulatory effect in the acute phase of inflammation and significant effect on the remodeling phase of tissue repair, also considering the important role that fibronectin plays in tissue structure regeneration.

Therefore we can support that Hilterapia can efficaciously promote tissue repair processes.

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